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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Which enzyme joins two DNA fragments?

Answer: DNA ligase.

  • A DNA ligase
  • B the DNA polymerase
  • C only a helicase
  • D a restriction one

Correct answer: A. DNA ligase

Explanation: DNA ligase seals fragments by forming phosphodiester bonds.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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