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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Palindromic sequences that are recognised by restriction enzymes read the same:

Answer: In the same orientation on both strands when read 5 to 3.

  • A When read in one direction along a single strand only
  • B On the same strand twice
  • C Only after being cut
  • D In the same orientation on both strands when read 5 to 3

Correct answer: D. In the same orientation on both strands when read 5 to 3

Explanation: A palindrome in DNA is a sequence of base pairs that reads the same on the two strands when the orientation of reading is kept the same (5 to 3).

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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