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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Restriction enzymes are used to:

Answer: Cut DNA at specific sequences.

  • A Join separate DNA fragments back together
  • B Cut DNA at specific sequences
  • C Copy DNA strands during replication
  • D Translate mRNA into a protein chain

Correct answer: B. Cut DNA at specific sequences

Explanation: Restriction enzymes (endonucleases) cut DNA at specific recognition sequences. They are molecular scissors for genetic engineering.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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