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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Which of the following is used to make the bacterial cell wall competent to take up DNA in transformation?

Answer: Divalent calcium ions.

  • A Glucose solution
  • B Distilled water
  • C Divalent calcium ions
  • D Sodium chloride crystals

Correct answer: C. Divalent calcium ions

Explanation: Treating cells with a specific concentration of a divalent cation such as calcium increases the efficiency with which DNA enters the bacterium through pores in its wall.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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