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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

The restriction enzyme EcoRI was isolated from which organism?

Answer: Escherichia coli.

  • A Escherichia coli
  • B Bacillus amyloliquefaciens
  • C Haemophilus influenzae
  • D Thermus aquaticus

Correct answer: A. Escherichia coli

Explanation: The name EcoRI comes from Escherichia coli RY13, the bacterium from which this restriction endonuclease was isolated.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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