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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

The cutting of DNA by restriction endonucleases followed by separation of fragments is visualised using:

Answer: Gel electrophoresis.

  • A Gel electrophoresis
  • B PCR
  • C Autoclaving
  • D Centrifugation

Correct answer: A. Gel electrophoresis

Explanation: DNA fragments produced by restriction digestion are separated and visualised by agarose gel electrophoresis.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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