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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

The separated DNA bands are cut out from the gel and extracted from the gel piece in a process known as:

Answer: Elution.

  • A Blotting
  • B Ligation
  • C Elution
  • D Annealing

Correct answer: C. Elution

Explanation: The process of extracting the separated DNA fragment from the cut-out gel piece is called elution; these purified fragments are then used in cloning.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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