Answer: 94-95 C.
- A 37 C
- B 55 C
- C 72 C
- D 94-95 C
Correct answer: D. 94-95 C
Explanation: PCR denaturation step: DNA is heated to ~94-95 degrees C to separate double-stranded DNA into single strands.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
Read the full Biotechnology: Principles and Processes notes →