Answer: Guide RNA (gRNA) complementary to the target DNA sequence.
- A Protein-protein interactions with transcription factor complexes
- B Guide RNA (gRNA) complementary to the target DNA sequence
- C Restriction enzymes that recognize specific palindromic sequences
- D Diffusible chemical signals released by the target cell
Correct answer: B. Guide RNA (gRNA) complementary to the target DNA sequence
Explanation: CRISPR-Cas9: a guide RNA (gRNA) is designed to match the target DNA sequence. It directs the Cas9 nuclease to cut at that specific location.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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