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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Sticky ends are produced by which class of enzymes?

Answer: Restriction enzymes.

  • A Restriction enzymes
  • B the DNA polymerases
  • C only ligase types
  • D a helicase group

Correct answer: A. Restriction enzymes

Explanation: Restriction enzymes leave overhanging sticky ends.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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