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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Somatic cell nuclear transfer (SCNT) was the technique used for:

Answer: Cloning Dolly the sheep.

  • A Making the first GMO crop
  • B Cloning Dolly the sheep
  • C Producing monoclonal antibodies
  • D Making recombinant insulin

Correct answer: B. Cloning Dolly the sheep

Explanation: Dolly the sheep (1996) was cloned by SCNT: nucleus from an adult mammary gland cell inserted into enucleated egg cell, then implanted in surrogate ewe.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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