Answer: Gene expression profiling of individual cells, revealing cellular heterogeneity and cell types within tissues.
- A Calculating a single averaged gene expression value across an entire bulk tissue sample collected
- B Gene expression profiling of individual cells, revealing cellular heterogeneity and cell types within tissues
- C Analysis that is restricted mainly to bulk tissue homogenates, and rarely individual single cells directly
- D Profiling restricted mainly to overall protein expression levels, rather than mRNA transcript abundance
Correct answer: B. Gene expression profiling of individual cells, revealing cellular heterogeneity and cell types within tissues
Explanation: scRNA-seq: RNA from individual cells is captured, barcoded, and sequenced. Reveals transcriptome of each cell. Identifies rare cell types, transitions, trajectories, and heterogeneity invisible in bulk sequencing.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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