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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Ligating an insert into a plasmid needs matching?

Answer: Sticky ends.

  • A Sticky ends
  • B the blunt long ends
  • C only the promoters
  • D a marker regions

Correct answer: A. Sticky ends

Explanation: Complementary sticky ends allow the insert to join the vector.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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