🔬 Biology · Biotechnology: Principles and Processes · NEET

In the plasmid pBR322, insertion of foreign DNA into the tetracycline-resistance gene inactivates it. This loss of a trait used to detect recombinants is called:

Answer: Insertional inactivation.

A Insertional inactivation
B Genetic transformation
C Transduction
D Conjugation

Correct answer: A. Insertional inactivation

Explanation: When foreign DNA is inserted within a marker gene such as tetracycline resistance, the gene is inactivated; this insertional inactivation lets recombinants be selected.

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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In the plasmid pBR322, insertion of foreign DNA into the tetracycline-resistance gene inactivates it. This loss of a trait used to detect recombinants is called:

Concept context

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