Answer: Primers bind to the complementary single strands.
- A The two DNA strands separate
- B The DNA is permanently denatured
- C Taq polymerase synthesises entirely new DNA strands
- D Primers bind to the complementary single strands
Correct answer: D. Primers bind to the complementary single strands
Explanation: After denaturation separates the strands, the temperature is lowered so that the two sets of primers anneal (hybridise) to the complementary regions of the single strands.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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