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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

In Southern blotting, which molecule is being detected?

Answer: Specific DNA sequences.

  • A RNA transcripts separated by gel electrophoresis
  • B Protein bands detected using a labeled antibody probe
  • C Specific DNA sequences
  • D Lipids extracted and separated by thin-layer chromatography

Correct answer: C. Specific DNA sequences

Explanation: Southern blot: DNA is separated by gel electrophoresis, transferred to membrane, then hybridized with a labeled DNA probe to detect specific sequences.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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