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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

In PCR, which enzyme extends the primers?

Answer: Taq polymerase.

  • A Taq polymerase
  • B the DNA ligase used
  • C only a restriction
  • D a helicase enzyme

Correct answer: A. Taq polymerase

Explanation: Thermostable Taq polymerase extends primers in PCR.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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