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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

During gel electrophoresis the separated DNA bands cannot be seen directly. They are visualised by staining with a dye and then exposing the gel to:

Answer: UV radiation.

  • A Infrared light
  • B UV radiation
  • C X-rays
  • D Visible white light only

Correct answer: B. UV radiation

Explanation: DNA separated on an agarose gel is stained with ethidium bromide and exposed to UV radiation, under which the DNA bands appear as bright orange bands.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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