Answer: Their short single-stranded overhangs form hydrogen bonds with complementary ends.
- A They have no phosphate groups
- B They are always much longer DNA fragments than blunt ends and bind far faster
- C Their short single-stranded overhangs form hydrogen bonds with complementary ends
- D They do not need DNA ligase
Correct answer: C. Their short single-stranded overhangs form hydrogen bonds with complementary ends
Explanation: Sticky ends have short single-stranded overhangs that base-pair (hydrogen bond) with complementary overhangs, holding the fragments together so DNA ligase can seal them easily.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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