Zaymiey

🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

Biosafety level 4 (BSL-4) laboratories are required for:

Answer: Work with dangerous pathogens with no treatment or vaccine (Ebola, Marburg, Nipah virus) that are transmitted by aerosol.

  • A Routine, everyday research involving common, non-pathogenic laboratory bacterial strains generally used in routine practice
  • B Work with dangerous pathogens with no treatment or vaccine (Ebola, Marburg, Nipah virus) that are transmitted by aerosol
  • C Work that is limited mainly to the handling of hazardous laboratory chemical reagents in storage overall in most cases
  • D Culturing of mostly non-pathogenic fungal species for routine taxonomic study purposes generally under typical conditions

Correct answer: B. Work with dangerous pathogens with no treatment or vaccine (Ebola, Marburg, Nipah virus) that are transmitted by aerosol

Explanation: BSL-4: maximum containment for dangerous, exotic agents (Ebola, Marburg, smallpox). Full-pressure suits or class III biosafety cabinets. Researchers shower on exit. No established treatment.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

Read the full Biotechnology: Principles and Processes notes →