Answer: Single base mutations in specific genes (e.g., sickle cell, cystic fibrosis) by differential hybridization under stringent conditions.
- A Mainly large-scale chromosomal rearrangements, such as inversions and reciprocal translocations between non-homologous chromosomes
- B Single base mutations in specific genes (e.g., sickle cell, cystic fibrosis) by differential hybridization under stringent conditions
- C Mainly large, multi-exon gene deletions that are readily and easily detectable using ordinary standard PCR techniques
- D Mainly the mere presence of foreign viral DNA sequences that have become integrated within the host cell genome
Correct answer: B. Single base mutations in specific genes (e.g., sickle cell, cystic fibrosis) by differential hybridization under stringent conditions
Explanation: ASO hybridization: oligonucleotide probes designed to match wild-type or mutant allele. Under stringent conditions (Tm), only perfect match hybridizes. Used for point mutation diagnosis.
Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.
Concept context
Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.
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