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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

After a recombinant protein is produced in a bioreactor, it must undergo downstream processing before marketing. This stage involves:

Answer: Separation, purification and formulation of the product.

  • A Inserting the gene into the vector
  • B Separation, purification and formulation of the product
  • C Cutting the DNA with specific restriction enzymes
  • D Amplifying the gene by PCR

Correct answer: B. Separation, purification and formulation of the product

Explanation: Downstream processing includes separation and purification of the product followed by formulation with preservatives and quality control testing before it is marketed.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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