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🔬 Biology  ·  Biotechnology: Principles and Processes  ·  NEET

A good cloning vector must have an origin of replication (ori). What is the role of this sequence?

Answer: It controls copy number and initiates replication.

  • A It codes for a selectable marker
  • B It binds the ribosome to start translation of the gene
  • C It cuts the DNA at specific sites
  • D It controls copy number and initiates replication

Correct answer: D. It controls copy number and initiates replication

Explanation: The ori is the sequence where replication starts; any linked DNA replicates from it, and it also governs the copy number of the vector in the host.

PlasmidGene of interestcutrestriction enzymeligateRecombinantplasmidHost cell

Recombinant DNA technology: a gene of interest and plasmid are cut by the same restriction enzyme, ligated into a recombinant plasmid, and introduced into a host cell.

Concept context

Recombinant DNA, GMOs, PCR, CRISPR, and biotechnology applications. Growing importance in NEET.

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