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🧪 Chemistry  ·  Biomolecules  ·  NEET & JEE

Sanger reagent (2,4-dinitrofluorobenzene) is used in protein analysis to identify the:

Answer: N-terminal amino acid.

  • A C-terminal amino acid
  • B Disulphide bridge position
  • C N-terminal amino acid
  • D Total number of residues

Correct answer: C. N-terminal amino acid

Explanation: Sanger reagent reacts with the free alpha-amino group at the N-terminus of a polypeptide, allowing identification of the N-terminal amino acid.

Peptide Bond Formation (Condensation)H₂N-CH(R₁)-C(=O)OHAmino acid 1+HNH-CH(R₂)-COOHAmino acid 2H₂N-CH(R₁)-CO-NH-CH(R₂)-COOHDipeptide+ H₂OThe -OH (from acid 1) and -H (from amine 2) combine to release water, forming the -CO-NH- peptide bond

Two amino acids join via a condensation reaction: the carboxyl -OH of one and an amine -H of the other are eliminated as water, leaving a peptide bond (-CO-NH-) linking them into a dipeptide; repeating this builds a full protein chain.

Concept context

The chemistry of life: carbohydrates, proteins, lipids, nucleic acids, enzymes, and vitamins. Understand their structures, classification, and biological functions. Highly relevant for NEET and Class 12 board exams.

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