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What is the role of the enzyme nitrogenase in nitrogen fixation?

Answer: It converts atmospheric N 2 to NH 3 ; requires anaerobic conditions and ATP.

  • A It converts nitrate ions back into ammonia within specialized root cells
  • B It converts atmospheric N<sub>2</sub> to NH<sub>3</sub>; requires anaerobic conditions and ATP
  • C It oxidizes ammonia into nitrite ions during the nitrification process
  • D It breaks down amino acids into ammonia during microbial decomposition

Correct answer: B. It converts atmospheric N<sub>2</sub> to NH<sub>3</sub>; requires anaerobic conditions and ATP

Explanation: Nitrogenase is the enzyme complex that reduces N<sub>2</sub> to NH<sub>3</sub> (N<sub>2</sub> + 8H+ + 8e- + 16ATP → 2NH<sub>3</sub> + H<sub>2</sub> + 16ADP + 16Pi). It is irreversibly inhibited by O<sub>2</sub>. Legume nodules maintain low O<sub>2</sub> using leghemoglobin.

Nitrogen Cycle: Key ConversionsN₂ (air)N-fixation(Rhizobium, nitrogenase)NH₃/NH₄⁺nitrificationNO₃⁻denitrificationback to N₂leghaemoglobin protectsnitrogenase from O₂

Atmospheric N₂ is fixed into ammonia by nitrogen-fixing bacteria (protected from oxygen by leghaemoglobin in root nodules), converted to nitrate via nitrification for plant uptake, and eventually returned to the atmosphere as N₂ via denitrification, completing the cycle.

Concept context

Essential mineral elements, their roles and deficiency symptoms. Nitrogen fixation and soil nutrition.

Read the full Mineral Nutrition notes →