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What is the role of chaperone proteins?

Answer: Assist in proper protein folding and prevent aggregation.

  • A Target and degrade misfolded proteins via the ubiquitin-proteasome pathway
  • B Assist in proper protein folding and prevent aggregation
  • C Actively translocate completed proteins across the ER and mitochondrial membranes
  • D Initiate assembly of the ribosomal subunits at the start codon

Correct answer: B. Assist in proper protein folding and prevent aggregation

Explanation: Molecular chaperones (e.g., HSP70, HSP90) bind to exposed hydrophobic regions of newly synthesized or stress-denatured proteins, preventing misfolding and aggregation, and facilitate correct folding.

Enzyme Kinetics: Rate vs Substrate Concentration[Substrate]Reaction rateVmaxKm½VmaxCurve flattens at high [S]: ALL enzyme active sites are occupied (saturation)

As substrate concentration rises, reaction rate increases steeply at first, then plateaus at Vmax once every enzyme molecule is working at full capacity; Km is the substrate concentration giving half-maximal rate, and a LOWER Km means the enzyme reaches that rate with less substrate (higher affinity).

Concept context

Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.

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