Answer: Type II cuts within or near recognition site; Type I cuts away from it.
- A Type I cuts within the recognition site; Type II cuts elsewhere
- B Type II cuts within or near recognition site; Type I cuts away from it
- C Type I requires ATP; Type II mainly requires Mg<sup>2+</sup> instead
- D Type II works mainly on RNA substrates specifically
Correct answer: B. Type II cuts within or near recognition site; Type I cuts away from it
Explanation: Type II restriction enzymes (used in genetic engineering) cut within or very close to their recognition sequence, producing predictable fragments. Type I enzymes cut far from their recognition site, making them impractical for cloning.
As substrate concentration rises, reaction rate increases steeply at first, then plateaus at Vmax once every enzyme molecule is working at full capacity; Km is the substrate concentration giving half-maximal rate, and a LOWER Km means the enzyme reaches that rate with less substrate (higher affinity).
Concept context
Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.