Answer: Lineweaver-Burk plot.
- A Arrhenius plot
- B Lineweaver-Burk plot
- C Ramachandran plot
- D Henderson-Hasselbalch plot
Correct answer: B. Lineweaver-Burk plot
Explanation: The Lineweaver-Burk (double reciprocal) plot (1/v vs 1/[S]) linearizes the Michaelis-Menten equation, allowing accurate determination of Km and Vmax from the graph.
As substrate concentration rises, reaction rate increases steeply at first, then plateaus at Vmax once every enzyme molecule is working at full capacity; Km is the substrate concentration giving half-maximal rate, and a LOWER Km means the enzyme reaches that rate with less substrate (higher affinity).
Concept context
Carbohydrates, proteins, lipids, nucleic acids, and enzyme kinetics. Essential foundation for understanding metabolism.